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Overview

The Veyra Biofoundry (VBF) opened in 2019 as a dedicated platform for high-throughput biological and chemical experimentation. Unlike the Institute's other core facilities, the Biofoundry operates primarily as an operator-run service: clients define their assay or synthesis requirements, and Biofoundry scientists design and execute the workflow using the facility's robotic platforms. Self-access is available to trained internal users only.

The facility houses three Hamilton STAR liquid-handling robots, an automated compound storage and retrieval system (2 °C and −20 °C stores), a BioTek microplate reader stack with absorbance, fluorescence, and luminescence detection, and an Echo 655 acoustic dispenser for nanolitre-volume compound transfers. The laboratory information management system (LIMS) tracks samples, reagents, and results throughout each workflow, and all data are delivered to clients in structured electronic formats.

The Biofoundry is managed by Dr. Ingrid Walcott, with three automation scientists (Priya Anand, Seun Okafor, and Dr. Thibault Morand) responsible for assay development, robot programming, and data analysis. Clients are assigned a named scientist for each project who serves as the primary point of contact from intake through delivery.

Platforms & instrumentation

Principal platforms in the Veyra Biofoundry. All platforms are operated by Biofoundry scientists for external users.
PlatformManufacturer / ModelCapacity / throughputPrimary applications
Primary liquid handler (×2)Hamilton STAR 8-channel + 96-MPH96- and 384-well; 1 µL–1 mL pipetting range; on-deck incubator + plate hotel (50 plates)Serial dilutions, reagent dispensing, sample reformatting, cell seeding, hit picking
Microplate liquid handler (×1)Hamilton STAR 96-channel MPH384-well primary; 0.5 µL minimum volume; 15-plate deckHTS dispensing, compound addition, high-throughput dilution series
Acoustic liquid dispenserLabcyte Echo 6552.5 nL–10 µL per droplet; 384- and 1536-well capable; contactless; DMSO compounds up to 40%Compound dosing, nano-scale serial dilution, miniaturised HTS
Multimode plate reader stack (×2)BioTek Synergy Neo2 (×2)Absorbance 230–999 nm; fluorescence (Ex/Em); TRF; luminescence; FP; kinetics mode; 6- to 1536-well platesCell viability, ELISA, enzyme kinetics, reporter assays, cytotoxicity
Automated compound store (2 °C)SPT Labtech mosaic (2 °C)100,000 tube positions; barcode-tracked; automated pick-and-placeBiological sample storage, assay reagent management
Automated compound store (−20 °C)SPT Labtech mosaic (−20 °C)50,000 tube positions; barcode-tracked; integrated with LIMS for compound inventoryCompound library management, intermediate stock storage
Plate sealer / peelerBrooks PlateLoc Thermal + PeelOffFoil and film sealing; compatible with all standard SBS formatsPlate sealing for storage and transport; peeling before read steps
Centrifuge (plate)Beckman Coulter Allegra X-30RSBS-format plate buckets; 300–2000 × g; temperature control 4–40 °CCell pelleting, debris removal, spin-down before reading
CO₂ incubator stackLiCONiC STX220 (4 units)Humidity controlled; 37 °C ± 0.1 °C; 5% CO₂; capacity 50 SBS plates per unit; robot-integratedCell-based assay incubation, live-cell kinetic studies

Services offered

Assay development

We design and optimise new biochemical or cell-based assays in 96-, 384-, or 1536-well formats. Starting from a client's described biology, we select appropriate detection mode, optimise reagent concentrations, validate with reference compounds, and establish a Z' factor ≥ 0.5 before proceeding to screening. Clients receive an assay validation report with all optimisation data.

Typical timeline: 3–8 weeks depending on assay complexity and cell line availability.

High-throughput screening (HTS)

We run compound libraries (client-supplied or from the Veyra compound collection, ~12,000 compounds) against validated assays. Single-concentration screening is standard; dose-response confirmation of hits is performed in the same workflow. Robotics handle compound dispensing, incubation, detection, and data capture; scientists review data quality and deliver a hit list with statistical analysis.

Throughput: up to 10,000 compounds per day in 384-well format.

Cell-based assays

We perform cell culture, transfection, compound treatment, and endpoint or kinetic detection for cell-based assays using the integrated incubator stack and plate reader. Supported assay types include cytotoxicity (CellTiter-Glo, resazurin), reporter gene (luciferase, GFP), caspase activation, and label-free impedance monitoring. Client-supplied cell lines are accepted after mycoplasma testing.

BSL-2 cell work is accommodated; BSL-3 is not within current facility scope.

Automated synthesis workflows

We support parallel synthesis in microplate format for small-molecule libraries, including Ugi reactions, reductive aminations, and amide coupling. Synthesis steps are integrated with automated workup, solvent exchange, and concentration steps using on-deck evaporators. Product quality is monitored by LC-MS (in-house or via the Spectroscopy Core). Typical plate sizes: 24- to 96-compounds per run.

Contact biofoundry@veyra.example for feasibility discussion before committing to a project.

Sample management & reformatting

We provide compound library management, plate reformatting between different SBS formats, serial dilution series preparation, and daughter-plate generation from primary compound stores. Barcode tracking throughout ensures full chain of custody. Large reformatting projects (1,000+ plates) can be accommodated with advance scheduling.

Data & LIMS

All experimental data are captured in the Biofoundry LIMS (LabVantage 8.7) and delivered to clients as structured exports (CSV, Excel, or SD file). Raw plate-reader data, QC metrics (CV, Z', S/B), hit calls, and dose-response curves are included as standard. Data are archived in the LIMS for five years post-project; clients receive a copy at project close.

3
Hamilton STAR robots
96- and 384-well capable
10,000
Compounds/day
Throughput in 384-well HTS mode
12,000
Compound collection
Available for internal HTS campaigns
5 yr
Data retention
LIMS archive post-project